Asian Journal of Research and Review in Agriculture

Influence of NaCl and PEG6000 Stresses on the Mitotic Chromosomal Biology of Bread Wheat Genotypes

Abdallah A. Mahmoud

Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.

Galal, A. R. El- Sherbieny

Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.

E. Ismail

Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.

Shimaa M. Ali

Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.

Ali M. Ali

Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.

Nehal, G. Abdelaziz

Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.

Nada A. Asaad

Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.

Saleh A. Abuzaid

Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.

Mohamed A. Abdelraouf

Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.

Haitham, M. A. Elsayed *

Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.

*Author to whom correspondence should be addressed.

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Abstract

Cytogenetic studies were conducted to evaluate the effect of NaCl and PEG₆₀₀₀ doses on cell division and chromosomal aberrations during the germination of wheat (Triticum aestivum L.) genotypes. Cytotoxic effects of NaCl and PEG₆₀₀₀ were mainly observed in meristematic cells in all wheat genotypes. Both stresses had adverse effects on dividing cells in root tips of all wheat genotypes lead to induction of chromosome aberrations including micronuclei, multinuclei, sticky metaphase, c-mitosis, anaphase bridge and vagrant chromosome, which indicated their action on the mitotic spindle. Cytological examination revealed that both NaCl and PEG₆₀₀₀ doses causes positive clastogenic effect as eroded chromosomal surfaces. These data reveal that NaCl and PEG₆₀₀₀ doses were capable in interfering with chromosomes providing valuable information for understanding the effects of NaCl and PEG₆₀₀₀ stresses on cytogenetics of wheat plants.

Keywords: Chromosomal aberrations, meristematic cells of root-tips, sodium chloride (NaCl), Polythylen Glycol-6000 (PEG6000).

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