Field Survey and Identification of Nematodes Associated with Citrus Trees in Dalanj Area, Sudan
Eisa M. Ahmed
*
Department of Crop Protection, Faculty of Natural Resources & Environmental Studies, University of Kordofan, P.O.Box 160, Elobeid 51111, Sudan.
Nigood M. Omer
Department of Crop Protection, Faculty of Natural Resources & Environmental Studies, University of Kordofan, P.O.Box 160, Elobeid 51111, Sudan.
Taisser A. Almaleeh
Department of Plant Protection, Ministry of Production and Economic Resources, Sudan.
*Author to whom correspondence should be addressed.
Abstract
Nematodes associated with citrus trees were surveyed and identified. A survey was conducted during the 2015-2016 season in three locations in the Dalanj locality, South Kordofan state (Dalanj, Eltokma, and Elfarshaia). Soil samples were collected from around infected citrus trees (Lemon, Grapefruit, and Mandarin). The juveniles were extracted from the soil using the Bearman funnel technique. Identification of nematodes is basically done according to the nematode's body measurements. Botanical extracts were prepared using water extracts. The mortality rates of juveniles were recorded after 24, 48, and 72 hours. According to the morphological characteristics of the nematode, the species was found to be Xiphinema basiri. The mortality rates of juveniles increased with the increase in concentration and exposure time. The extracts from the bark of Punica grantum, seeds of Moringa oleifera, and seeds of Croton zambesias, were found to be extremely toxic to juveniles of Xiphinema basiri (35.33, 30.33, and 26.33%), respectively, after 72 hrs, at a concentration of 10.000 ppm, compared to the average mortality rates of juveniles treated with Cassia senna leaves and Moringa olifera (21and 19.67%). At 100ppm, all botanical extracts exhibited low mortality rates of 24.67-10% after 72 hrs, only the extract from the bark of Punica grantum at all exposure times showed mortality rates above 20%. All of the seed extracts were found to have better nematicidal activity than the leaf extracts.
Keywords: Nematodes, Dalanj locality, citrus trees, botanic extracts, Sudan
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References
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